001, respectively). These values were obtained using the following risk function: H(t) = [h0(t)]e(0.415X 5–1.012 X7-0.631 X8+1.552 X10+1.073X11) (Table 6). Figure 5 Kaplan-Meier survival curves for positive and negative expressions of Hsp90-beta and annexin A in lung cancer. (A) Among all 65 lung cancer cases, a higher expression of annexin A1 was associated with a longer post-surgery survival time (p = 0.014). (B) A higher expression of Hsp90-beta is also related to a longer post-surgery

survival time (p = 0.021). Table 6 Cox proportional hazards regression model analysis of disease-free survival Variables (X) Categories (different groups) P value OR value 95% CI for OR Lower Upper Gender (X1) Male (X1-0) Avapritinib ic50 vs. female (X1-1) 0.785 – - – Age (X2) <60 (X 2-0) vs. ≥60 (X 2-1) 0.492 - - - Smoking (X3) 0 (X3-0) vs. 0.1-40 (X3-1) vs. >40 (X3-2) 1.062 – - – Histology (X4) LAC (X4-0) vs. LSCC (X4-1) vs. SCLC (X4-2) MG-132 ic50 vs. LCLC (X4-3) 0.908 – - – Differentiation (X5) Poor (X5-0) vs. moderate (X5-1) vs. well (X5-2) 0.013 1.514 1.090 2.103 T stage (X6) T1-2 (X6-0) vs. T3-4 (X6-1) 0.769 – - – Lymphatic invasion (X7) Positive (X7-0) vs. negative (X7-1)

0.018 0.697 0.516 0.941 TNM (X8) I-II (X8-0) vs. Lorlatinib research buy III-IV (X8-1) 0.001 0.532 0.370 0.765 Pleural invasion (X9) Absent (X9-0) vs. Present (X9-1) 0.154 – - – Annexin A1 (X10) Low (X10-0) vs. moderate (X10-1) vs. high (X10-2) 0.000 4.723 2.703 8.253 Hsp90-beta (X11) Low (X11-0) vs. moderate (X11-1) vs. high (X11-2) 0.000 2.923 1.857 4.601 Imaging (X12) Central (X12-0)vs. ambient (X12-1) 1.600 – - – Risk function: H(t) = [h0(t)]e(0.415 X5 – 1.012 X7 – 0.631 X8 + 1.552

X10 + 1.073 X11) LAC, lung adenocarcinoma; LSCC, lung squamous cell carcinoma; SCLC, small cell lung cancer; LCLC, large cell lung cancer; Smoking, pack years of smoking. OR, odds ratio; CI, confidence interval. The relative risk (RR) for the expressions of Hsp90-beta Methane monooxygenase and annexin A1 in lung cancer Pearson’s χ 2-test was performed to evaluate RR associated with the expressions of Hsp90-beta and annexin A1 and lung cancer. The results indicated that the RR value for positive/negative expression of Hsp90-beta was 12.21 (p = 0.000) with a 95% confidence interval (CI) of 4.334 to 34.422. Statistical analysis results showed that subjects with higher Hsp90-beta expression exhibited a significantly higher risk for lung cancer development (RR = 12.21) compared with subjects with lower Hsp90-beta expression. The RR value of annexin A1 expression was 6.6 (p = 0.000), and the 95% CI was 2.415 to 18.04. This result indicated a higher risk for lung cancer development (RR = 6.6). The higher mRNA expression levels of Hsp90-beta and annexin A1 also indicated a higher risk for lung cancer development (RR = 16.25; RR = 13.33) compared with the protein expression (Table 7).