, 2007, Gordon and Waterhouse, 2007 and Mao et al., 2007). The toxicity was due to the dsRNA being transmitted from plant tissues to the insects by ingestion,

and then being further Talazoparib chemical structure processed in the animal into an siRNA that silenced one or more genes essential for life, or essential for detoxifying natural plant toxins (i.e., gossypol in cotton). Others have used direct feeding of dsRNA or dsRNA in liposomes as insecticides (Chen et al., 2010 and Whyard et al., 2009). As with the human studies discussed above, there is evidence of selective uptake of miRNAs from food. A feeding study of insects found that some small RNAs that were less abundant in the plant were more abundant in the insects that fed on the plant (Zhang et al., 2012b). It also found that insects which fed on dicots seemed to accumulate a miRNA that was more suggestive of a monocot origin. As a meta-analysis of small RNA datasets, this study could not confirm the purity of diets or exposure routes for animals (e.g., ingestion, inhalation, soaking through skin). The authors suggested that many or most detections of plant miRNAs in animals occurred via contamination from non-dietary sources. While PF-02341066 cell line this is important speculation, contamination does not sufficiently explain all the results. The contamination source proposed by the authors was from the mixture of plant and animal

small RNA pools combined during multiplex sequencing. This can occur because of the capacity of the DNA sequencers to run reactions in parallel. However, that conclusion seems at odds with regular detection by others of miRNAs of plant origin that are not the most abundant plant miRNAs and assumes that all datasets assembled by others would have had the same mixture of plant and animal libraries used by the authors of the transcriptomic survey (Zhang et al., 2012b). The regulatory framework

for GM crops in Australia and New Zealand consists of a shared food safety regulator called FSANZ. Under the Food Safety Act, FSANZ must approve as safe all foods derived from PJ34 HCl GMOs, following an assessment based on “internationally recognized scientific, risk-based methods” (p. 411 Brent et al., 2003). FSANZ uses information provided by the developer of the GMO, but also the scientific literature, advice from independent scientists and the evaluations of regulators from other countries (Brent et al., 2003 and Hansard, 2008). The Centre for Integrated Research in Biosafety (INBI) has argued within the regulatory framework of Australia/New Zealand that as part of the legislated requirement for safety testing of GMOs, any potential novel dsRNA molecules should be described and then evaluated for causing physiological effects in the GM plant or on any consumer of the plant, be that insects, wildlife or humans (Heinemann, 2009 and Heinemann et al., 2011).