4 index (normal, <0.8 index), and immunoglobulin G was >= 240 IU/mL (normal, <6 IU/mL). Maternal oral administration of azithromycin in addition to sulfadoxine, pyrimethamine and acetylspiramycin was conducted. Spontaneous vaginal delivery occurred at 32 weeks and a male infant weighing 2036 g was born. Hepatosplenomegaly, chorioretinitis, hydrocephalus, intracranial calcifications, ascites, and meningitis were confirmed NSC 136476 after birth. The infant underwent therapy with pyrimethamine and sulfadiazine. It seems imperative to establish a new drug choice for fetal therapy of severe symptomatic

toxoplasmosis in order to reduce the maternal and fetal risks of drug side-effects.”
“A cold-adapted lipase producing bacterium, designated SS-33(T), was isolated from sea sediment

collected from the Bay of Bengal, India, and subjected to a polyphasic taxonomic study. Strain SS-33(T) exhibited the highest 16S rRNA gene sequence similarity with Staphylococcus cohnii subsp. urealyticus (97.18 %), Staphylococcus saprophyticus subsp. bovis (97.16 %) and Staphylococcus cohnii subsp. cohnii (97.04 %). Phylogenetic analysis based on the 16S rRNA gene JIB-04 datasheet sequences showed that strain SS-33(T) belongs to the genus Staphylococcus. Cells of strain SS-33(T) were Gram-positive, coccus-shaped, non-spore-forming, non-motile, catalase-positive and oxidase-negative. The major fatty acid detected in strain SS-33(T) was JPH203 price anteiso-C15:0 and the menaquinone was MK-7. The genomic DNA G + C content was 33 mol%. The DNA-DNA hybridization among strain SS-33(T) and the closely related species indicated that strain SS-33(T) represents a novel species of the genus Staphylococcus. On the basis of the morphological, physiological and chemotaxonomic characteristics, the results of phylogenetic analysis and the DNA-DNA hybridization, a novel

species is proposed for strain SS-33(T), with the name Staphylococcus lipolyticus sp. nov. The strain type is SS-33(T) (=MTCC 10101(T) = JCM 16560(T)). Staphylococcus lipolyticus SS-33(T) hydrolyzed various substrates including tributyrin, olive oil, Tween 20, Tween 40, Tween 60, and Tween 80 at low temperatures, as well as mesophilic temperatures. Lipase from strain SS-33(T) was partially purified by acetone precipitation. The molecular weight of lipase protein was determined 67 kDa by SDS-PAGE. Zymography was performed to monitor the lipase activity in Native-PAGE. Calcium ions increased lipase activity twofold. The optimum pH of lipase was pH 7.0 and optimum temperature was 30 A degrees C. However, lipase exhibited 90 % activity of its optimum temperature at 10 A degrees C and became more stable at 10 A degrees C as compared to 30 A degrees C. The lipase activity and stability at low temperature has wide ranging applications in various industrial processes. Therefore, cold-adapted mesophilic lipase from strain SS-33(T) may be used for industrial applications.