6%) patients. About 40% of the patients had mediastinal lymph node metastases

at the time of surgery, classified as stage N1 and stage N2 in 43 (28.5%) and 18 (11.9%) patients, respectively. The CDK inhibition study comprised 64 cases of adenocarcinoma (ADC), 35 cases of large cell carcinoma (LCC), and 52 cases of squamous cell carcinoma (SCC) of the lung (Table 1). The median MET CN in tumor tissue was 2.05 (ranged from 0.50 to 7.40) and was not significantly affected by analyzed clinicopathologic variables. With 3.0 copies used as a cutoff in MET CN evaluation, gene copy gain was observed in 28 (18.5%) tumor samples, including 15 cases with 3.0 to 3.99 MET copies per cell and the remaining 13 samples containing from 4.0 to 7.7 copies ( Table 1). In our cohort PD332991 of patients with NSCLC, MET CNG was observed approximately 2.7- and 2.0-fold more frequently in the tumors with increased EGFR and HER2 CN compared to the tumors without the increase (P = .002 and .049 for EGRF and HER2, respectively) and about 2.4-fold more frequently in tumors harboring EGFR mutations compared to tumors with wild-type EGFR (P = .071). However, subgroup analysis for particular tumor histologic types revealed that statistically significant associations between MET CNG and EGFR or HER2 gene alterations occurred only in the ADC group but not in the LCC or SCC group. No associations

between MET CN and KRAS gene mutations or copy gain were found in particular histologic types of cancer ( Table 2). We were unable to determine MET cDNA in 16 analyzed tumor and/or normal lung tissue specimens and these paired samples were excluded from the assay. The MET mRNA level was significantly higher in tumor tissue as compared to unaffected tissue (relative quantity (RQ) geometric mean, 1.76; 95% confidence interval (CI), 1.29-2.40; P < .001). However, with respect to tumor histologic types, a statistically significant alteration was obtained only in ADCs

(RQ geometric mean, 2.14; 95% CI, 1.33-3.45; P < .001). No significant associations between MET mRNA expression and patients’ characteristics were found ( Table 1). Linear regression model revealed a statistically significant link between MET CN and mRNA expression in lung tumor tissue ( Figure 1). Gain of an additional DOCK10 gene copy resulted in 1.51-fold increase in the expression level (95% CI, 1.22-1.87; P < .001). During the follow-up period, 34.4% of the patients showed disease recurrence and most of them (31.8%) died. The median OS was 30 months (ranged from 2 to 86 months), and the DFS was 33 months (ranged from 2 to 85 months). In Kaplan-Meier curve analysis, neither MET CN alterations nor MET mRNA expression level influenced patients’ OS or DFS ( Figure 2, A and B). However, when the analysis was restricted to patients with ADC histology, both DFS and OS were shorter in the cases with an increased MET CN, although only DFS difference was statistically significant (log-rank test, P = .044 and P = .