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GR. London: Wolfe Publisher, Year Book Europe Limited; 1994:268–272. 25. American Veterinary Medical Association: U.S. Pet Ownership & Demographics Sourcebook. 2007. 26. Ipsos Reid : Paws and Claws, a syndicated study on Canadian Pet Ownership. 2001. 27. Lee DH, Zo YG, Kim SJ: Nonradioactive method to study genetic profiles of natural 3-MA chemical structure bacterial communities by PCR-single-strand-conformation polymorphism. Appl Environ Microbiol 1996,62(9):3112–3120.PubMed Authors’ contributions BC participated in sample collection, carried out all sample preparation and

testing, participated in statistical analysis and drafted the manuscript. MN coordinated sample collection and participated in the design of the study and analysis. JEH conceived of the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.”
“Background Tularemia is a zoonotic disease caused by the highly infectious, virulent, gram-negative bacterium F. tularensis. This bacterial disease occurs in various clinical forms depending on the route of inoculation and the virulence of the F. AZD1152 solubility dmso tularensis strain involved [1]. The geographical distribution of F. tularensis was long regarded to be restricted to the Northern Hemisphere [2], and only very recently F. tularensis-like strains have been cultured in Queensland, Australia [3], and Thailand, South-East Asia [4]. F. tularensis has a broad host range and can affect more animal species than any

other zoonotic pathogen [2]. Whereas human infections in North America are mainly due to tick bites or contact with rabbits, several enzootic cycles have been described in the Eurasia. Here, F. tularensis is often associated with water and aquatic fauna Ixazomib and its transmission is considered to be more complex involving blood-sucking arthropods like mosquitoes or ticks or direct contact with infected mammals [5, 6]. Due to its infectious nature, ease of dissemination and high case fatality rate especially in respiratory infection, F. tularensis was the subject in diverse military biological weapons programs and is still included among the top six agents with high potential to be misused in bioterrorism [7]. The taxonomic position of F. tularensis is complex and has changed frequently. At present, the Francisellacae family contains four validly published species: F. tularensis, F. novicida, F. noatunensis and F. philomiragia. F.