Fig. 1 and Fig. 2). Compared with placebo, administration of spironolactone significantly enhanced counts

of CD4+ T cells and their naïve subpopulation, with these effects concentrating on the early part of the night. For both populations the Condition × Early/late × Time interaction term revealed to be significant (total CD4+ T cells: F(3,30) = 3.50, p = 0.038; naïve CD4+ cells: F(3,30) = 3.41, p = 0.048). Moreover, post hoc pairwise comparisons showed that for both the total CD4+ population and the naïve CD4+ subset the spironolactone induced increase in cell counts was most consistent at 3:30 h (p = 0.003; 0.007, respectively). Similar increases after spironolactone in cell numbers of total T cells, central memory CD4+ and naïve CD8+ T cells did AZD6244 datasheet not reach significance in the ANOVA results (F(3,30) = 2.95, p = 0.061; F(3,30) = 2.33, p = 0.107;

selleck compound F(3,30) = 2.78, p = 0.072, respectively, for Condition × Early/late × Time; p = 0.010; 0.028; 0.066, respectively, for post hoc pairwise comparisons at 3:30 h). All other subpopulations (total CD8+ T cells, central memory CD8+ T cells, and all CD62L− subsets) were not influenced by spironolactone ( Fig. 1 and Fig. 2). Spironolactone did not influence the expression of CXCR4 on any subpopulation, nor did it affect the time course of CXCR4 expression. The same was true for the expression of CD62L (data not shown). CXCR4 expression was highest in the naïve and central memory subpopulations of CD4+ and CD8+ T cells, and showed a decline over time during the first night half reaching lowest levels around 3:30 h. Thereafter, expression continuously increased during the late night on naïve CD4+ and CD8+ T cells as well as on central memory and effector memory CD4+ T cells (F(3,30) ⩾ 5.56, p ⩽ 0.012, for respective Time and Early/late × Time effects, data not shown). Plasma cortisol showed the typical circadian variation peaking at the time of awakening (Fig. 3). Levels of aldosterone and ACTH old showed a similar time course, both peaking at 8:00 h. Spironolactone enhanced cortisol levels at 9:30 h compared

with the placebo condition (F(1,10) = 7.72, p = 0.020, for Condition × Early/late interaction; p = 0.026 for post hoc pairwise comparison), whereas ACTH levels were not affected by the MR blocker. This pattern is well in line with previous studies ( Dodt et al., 1993 and Young et al., 1998) which likewise found that MR antagonists increased cortisol in the absence of changes in ACTH. Increases in aldosterone levels after spironolactone administration did not reach significance (F(3,30) = 3.00, p = 0.073, for Condition × Early/late × Time interaction; p = 0.033 and 0.093 for post hoc pairwise comparisons at 3:30 and 6:30 h, respectively). Noradrenaline and adrenaline were not influenced by spironolactone. We also calculated a ratio between aldosterone and cortisol because cortisol has an influence on lymphocyte migration which could compete with that of aldosterone.