In conclusion, we believe that previous studies proposing EMT as a significant source for collagen-producing myofibroblasts in the liver are limited by the approach, methodology, and quality of data. These studies rely on double-immunofluorescence staining of epithelial markers and surrogate markers for EMT but lack functional evidence for ECM production by hepatocytes-derived cells. Our study clearly identifies collagen-expressing cells, but none of which are derived from hepatocytes in vivo. Although we recognize the limitation of our study, our results strongly challenge
the concept that hepatocytes in vivo acquire a mesenchymal phenotype through EMT to produce ECM in liver Buparlisib nmr fibrosis. Additional Supporting Information may be found in the online version of this article. “
“Aim: In spite of numerous studies on the association between diabetes mellitus (DM) and hepatocellular carcinoma (HCC), the results are inconsistent and whether and how the effect of DM on the risk for HCC is PI3K Inhibitor Library modified or synergistically exerted by hepatitis virus infection are still unclear. We aimed to elucidate and quantify the effect modification and synergism between hepatitis B and C virus (HBV and HCV, respectively) and DM leading to the risk for HCC and also assess the independent contribution of DM to the risk for HCC at population level (population attributable fraction) in a high prevalence area of hepatitis virus infection. Methods: A hospital-based
case–control study was conducted from one medical center. Information on hepatitis B and C virus infection and DM status
(defined by 8-h fasting blood glucose level ≥126 mg/dL, current use of oral hyperglycemic agent or insulin injection) was collected to assess interaction of hepatitis virus infection with DM on the risk for HCC. Results: selleck chemical The association between DM and the risk for HCC was significant regardless of the presence of HBV infection, whereas a significant positive association was noted for HCV negativity. Synergistic interactions between DM and HBV infection were significant. In the absence of both hepatitis virus infections, the independent effect of DM accounted for 7.5% risk for HCC from the underlying population. Conclusion: The effect of DM on the risk for developing HCC is higher in HCV negative patients and synergistic with HBV infection. The independent effect of DM provides a new insight to the prevention of HCC other than virus-related mechanism. “
“The impact of viral status on recurrence of hepatitis B-related hepatocellular carcinoma (HCC) after curative therapy remains controversial. This meta-analysis aimed to determine whether the presence of viral load, genotype, specific mutation and antiviral therapy influenced HCC recurrence after curative therapy. We performed a meta-analysis including 20 studies to assess the effect of viral status and antiviral therapy with nucleoside analog on recurrence of HCC after curative therapy.