smegmatis glmM gene knockdown strain. PLoS One 2013,8(4):e61589.PubMedCentralPubMedCrossRef 24. Tafelmeyer P, Laurent C, Lenormand P, Rousselle JC, Marsollier
L, Reysset G, Zhang R, Sickmann A, Stinear TP, Namane A, Cole S: Comprehensive proteome analysis of Mycobacterium ulcerans and quantitative comparison of mycolactone biosynthesis. this website Proteomics 2008,8(15):3124–3138.PubMedCrossRef 25. Blair DE, van Aalten DM: Structures of Bacillus subtilis PdaA, a family 4 carbohydrate esterase, and a complex with N-acetyl-glucosamine. FEBS Lett 2004,570(1–3):13–19.PubMedCrossRef 26. Bui NK, Turk S, Buckenmaier S, Stevenson-Jones F, Zeuch B, Gobec S, Vollmer W: Development of screening assays and discovery of initial inhibitors of pneumococcal peptidoglycan deacetylase PgdA. Biochem Pharmacol 2011,82(1):43–52.PubMedCrossRef 27. Leal AF, de Lima Neto RG, Macedo DP, Beltrao EI, Neves RP: Carbohydrate profiling of fungal cell wall surface glycoconjugates of Trichophyton
tonsurans and other keratinophilic filamentous fungi using lectins. Mycoses 2011,54(6):e789-e794.PubMedCrossRef selleck compound 28. Kaoukab-Raji A, Biskri L, Bernardini ML, Allaoui A: Characterization of SfPgdA, a Shigella flexneri peptidoglycan deacetylase required for bacterial persistence within polymorphonuclear neutrophils. Microbes Infect 2012,14(7–8):619–627.PubMedCrossRef 29. Psylinakis E, Boneca IG, Mavromatis K, Deli A, Hayhurst E, Foster SJ, Varum KM,
Bouriotis V: Peptidoglycan N-acetylglucosamine deacetylases from Bacillus cereus, highly conserved proteins in Bacillus anthracis . J Biol Chem 2005,280(35):30856–30863.PubMedCrossRef 30. Milani CJ, Aziz RK, Locke JB, Dahesh S, Nizet V, Buchanan JT: The novel polysaccharide deacetylase homologue Pdi contributes to virulence of the aquatic pathogen Streptococcus iniae . Microbiology 2010,156(Pt 2):543–554.PubMedCentralPubMedCrossRef Competing interests The authors declare that they have no competing interests. Authors’ contributions SY constructed expression vectors, prepared Rv1096 protein and conducted lysozyme susceptibility assays, deacetylase activity assays, as well as prepared this manuscript. 3-mercaptopyruvate sulfurtransferase FZ purified Rv1096 protein and determined kinetic parameters of PG deacetylase. JK performed bioinformatic analyses of Rv1096 with known PG deacetylases. WZ performed bioinformatic analysis of Rv1096 and the statistical analyses. GD prepared samples for acid-fast staining and SEM. YX participated in designing experiments of the study. YM proposed this project, designed most of experiments and prepared this manuscript. All authors read and approved the final manuscript.”
“Background Acinetobacter baumannii is a non-fermentative Gram-negative bacterium that has emerged as a troublesome opportunistic human pathogen associated with life-threatening infections in the immunocompromised and critically ill [1].