The fitted curves in Fig. 4 for the membrane-bound RCs are obtained using analysis Method 2. The measured and fitted bleaching kinetics for several samples of isolated RCs with Triton X-100 and LDAO, and for membrane-bound RCs, are summarized Stem Cell Compound Library supplier in Table 2. Fig. 2 Bleaching kinetics of Triton X-100 isolated RCs after turning on CW illumination for a 2-second time interval. The transmittance at a wavelength of 865 nm, T 865, versus time is shown. The smooth line shows the results of fitting using

Method 1 (top graph) and Method 2 (bottom graph) Fig. 3 Bleaching kinetics of LDAO isolated RCs after turning on CW illumination for a 2-second time interval. The transmittance at a wavelength of 865 nm, https://www.selleckchem.com/products/PLX-4032.html T 865, versus time is shown. The smooth line shows the results of fitting using Method 1 (top graph) and Method 2 (bottom graph) Fig. 4 Bleaching kinetics of membrane bound RCs after turning on CW illumination

for a 2-second time interval. The transmittance at a wavelength of 865 nm, T 865, versus time is shown. The smooth line shows the results of fitting using Method 2 Table 2 Summary of the light intensity parameter and effective recombination rate constant values for isolated and membrane-bound RCs Sample α m1 mW−1 cm2 s−1 (uncertainty) α m2 mW−1 cm2 s−1 (uncertainty) \( k^\prime_\textrec , \) s−1 (uncertainty) \( k_A , \) s−1 (uncertainty) \( k_B , \) s−1 (uncertainty) C A arb. un. (uncertainty) C B arb. un. (uncertainty) LDAO 0.8180 (0.0004) 0.8171 (0.0006) 1.056 (0.001) 8.29 (0.24) 0.758 (0.005) 0.0280 [0.23] (0.0002) 0.0914 [0.77] (0.0004) Triton X-100 0.965 (0.001) 0.979 (0.002) 4.491 (0.008) 7.92 (0.12) 1.49 (0.05) 0.217 [0.78] (0.002) 0.059 [0.22] (0.002) Membranes 8.72 (0.02) 6.30 (0.02) 0.817 (0.005) 18.36 (0.89) 0.22 (0.01) 0.046 (0.54) (0.001) 0.0386 (0.46) (0.0003) α m1 and α m2 are the light intensity conversion parameters obtained

experimentally using Method 1 and Method 2, respectively. \( k^\prime_\textrec \) is the charge recombination rate obtained using analysis Method 2, and Baf-A1 order k A and k A are the charge recombination rates obtained using analysis Method 1. C A and C B are the relative proportions of Q B -depleted and Q B -enriched RCs in the sample, respectively. The values in square brackets next to C A and C B are the normalized portions of Q B -depleted and Q B -active RCs. The values in parenthesis underneath the measured values are the uncertainties for those measurements The light intensity values used for I exp are the estimated excitation intensities at the middle of the sample cuvette and are determined separately for each sample trial. First, the excitation intensity at the incident surface of the cuvette is measured.