These strains were originally isolated from the oral cavities of subjects with various forms of click here Periodontal disease; who resided in China, Japan, the Netherlands, Canada or the USA. We subjectively chose these particular strains based on several main criteria: 1) their diverse geographical origin; 2) their inclusion in one or more previously-published scientific investigations; and 3) their reported differences in phenotypic properties. Using the genome sequence of the type strain (ATCC 35405), seven protein-encoding genes distributed throughout the

single, circular chromosome were selected for genetic analysis: flaA, recA, pyrH, ppnK, dnaN, era and radC (see Table 2). This approach enabled us to obtain a representative snapshot of genomic composition within each strain. None of these genes are predicted selleck compound to reside in regions of suspected prophage origin [18]. Using a PCR-based strategy, the full length gene sequences for all seven genes were determined for each of the 19 other T. denticola strains. Details are shown in Table 3. Only the era

gene from the ATCC 700768 strain could not be PCR-amplified using any primer set, and its sequence was determined by direct sequencing of purified chromosomal DNA. The gene sequences corresponding to the major rRNA component of the small ribosomal subunit (rrs, 16S rRNA) were also determined for each

strain, to confirm their taxonomic assignment. In T. denticola, 16S Ipatasertib solubility dmso rRNA is encoded by two genes (rrsA, rrsB), which have identical sequences and are positioned at distinct chromosomal loci (see Table 2) [18]. Table 1 Origins of the Treponema denticola strains used in this study Strain Origin Disease /isolation site(depositor) Thiamine-diphosphate kinase Reference ATCC 35405T (strain a) Canada Periodontal pocket (ECS Chan) [30] ATCC 35404 (strain c, TD-4) Canada Periodontal pocket (ECS Chan) [30] ATCC 33521 (strain 11) USA Subgingival plaque (RK Nauman) [31] ATCC 33520 (strain W) USA Subgingival plaque (RK Nauman) [31] GM-1 USA Human periodontal pocket (SC Holt) [32] MS25 USA Human periodontal pocket (SC Holt) [32] ST10 USA (S. Socransky) [33, 34] CD-1 USA (WJ Loesche) – OTK USA (RC Johnson) – OT2B USA (RC Johnson) – NY535 Netherlands Gingival biopsy of human periodontitis (FHM Mikx) [35–37] NY545 Netherlands Gingival biopsy of human periodontitis (FHM Mikx) [36, 37] NY531 Netherlands Gingival biopsy of human periodontitis (FHM Mikx) [36, 37] NY553 Netherlands Gingival biopsy of human periodontitis (FHM Mikx) [36, 37] ATCC 700771 (OMZ 834) China Chinese ANUG patient (C. Wyss) [15] ATCC 700768 (OMZ 830) China Chinese ANUG patient (C. Wyss) [15] OMZ 852 China Chinese ANUG patient (C. Wyss) [15] OMZ 853 China Chinese gingivitis patient (C. Wyss) – S2 Japan (T. Eguchi) [38] OKA3 Japan (T.