Fermentation factors play an important role in product enhancement. Hence, glutaminase production Entinostat manufacturer was optimized using an artificial neural network (ANN) coupled genetic algorithm (GA).
RESULTS: A ’6-12-1′ topology ANN was constructed to identify the nonlinear relationship between fermentation factors and enzyme yield. ANN-predicted values were optimized for glutaminase production using a GA. The overall mean absolute predictive error (MAPE) and the mean square errors (MSE) were observed to be 0.00125 and 1.77 and 0.002 and 3.06 for training and testing, respectively. The goodness of neural network prediction (coefficient
of R(2)) was found to be 0.996. The maximum interactive impact on glutaminase production was noted with rpm versus medium volume. The use of ANN-GA hybrid methodology resulted in a significant improvement (47%) in glutaminase yield.
CONCLUSION: Five different
optimum fermentation conditions out of 500 revealed maximum enzyme production. Glutaminase enzyme production in this Bacillus subtilis RSP-GLU is strongly influenced by aeration of the fermentation. A hybrid ANN-GA effectively identifies the different fermentation conditions for optimum production of enzyme in a given large set of conditions. (C) 2009 Society of Chemical Industry”
“Objectives: Surgical treatment of carotid stenosis after the onset of ischaemic symptoms should be performed within 2 weeks.
This aim was accomplished only in 11% during the years 2007-2008 in the Helsinki University Central Hospital (HUCH) region. Since then, special efforts have Angiogenesis inhibitor been made in order to shorten the delay. The aim of this study was to find out how these changes affected the symptom-to-knife time (SKT).
Materials and methods: All symptomatic patients (n = 144) who had carotid endarterectomy (CEA, n = 145) in HUCH in 2010 were retrospectively analysed and the SKT was determined.
Results: Of the operations, 37% (n = 53) were performed Selleck LY2835219 within the recommended 2 weeks. The median SKT was 19 days (1-183). Of the patients who came to HUCH on an emergency basis (n = 80), 55% (n = 45) were operated within 2 weeks and their median SKT was 13 days (1-148).
Conclusions: The changes that were made in 2008-2009 have significantly shortened the delay in the treatment of carotid stenosis, but the desired time frame of 2 weeks was reached far too seldom. The greatest benefit from preventive CEA is achieved when patients are referred emergently to a clinic where neurologist, imaging resources and vascular surgeon are available. (C) 2012 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.”
“BACKGROUND: A generalized methodology for the synthesis of a hybrid controller for affine systems using sequential adaptive networks (SAN) is presented.